mitoxpress xtra oxygen-sensitive fluorescent probe (Agilent technologies)
Agilent technologies is a verified supplier 
Agilent technologies manufactures this product 
90
Structured Review
Agilent technologies
mitoxpress xtra oxygen-sensitive fluorescent probe
Mitoxpress Xtra Oxygen Sensitive Fluorescent Probe, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitoxpress+probe/pmc10594633-336-11-17
Average 90 stars, based on 1 article reviews
Mitoxpress Xtra Oxygen Sensitive Fluorescent Probe, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitoxpress+probe/pmc10594633-336-11-17
Average 90 stars, based on 1 article reviews
mitoxpress xtra oxygen-sensitive fluorescent probe - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "A critical role for staphylococcal nitric oxide synthase in controlling flavohemoglobin toxicity"
Article Title: A critical role for staphylococcal nitric oxide synthase in controlling flavohemoglobin toxicity
Journal: Redox Biology
doi: 10.1016/j.redox.2023.102935
Figure Legend Snippet: seNOS-derived NO 2 − activates respiration to sustain growth . A. Aerobic growth of the WT and Δ nos mutant was measured spectrophotometrically (OD 600 ) following incubation at 37 °C and 250 rpm. B. The glucose, ( C ) lactate and ( D ) acetate concentrations were determined from culture supernatants at the indicated times (mean ± SD, n = 3). E. The intracellular redox ratio (NADH/NAD + ) was measured during the exponential growth phase of strains (mean ± SD, n = 3, Student's t -test; *** P ≤ 0.0005). F. Gentamicin susceptibility assay. The ratio of the area under the growth curve (AUC) of cultures challenged with 2 μg/ml gentamicin relative to cultures grown in the absence of antibiotic (referred here as relative growth) was determined as the measure of gentamicin sensitivity of bacterial strains (mean ± SD, n = 3, Two-way ANOVA with Sidak's post-comparison test; * P ≤ 0.05). The Δ qox mutant (cytochrome oxidase mutant) with impaired respiration was included as a positive control in the assay. NaNO 2 concentration, 1 mM. G. Oxygen consumption was determined by measuring the time-resolved fluorescence of the oxygen-sensitive probe (MitoXpress, Luxcell Biosciences) in cultures supplemented with or without 1 mM NaNO 2 (mean ± SD, n = 3, Two -way ANOVA with Sidak's post-comparison test; **** P ≤ 0.00005). H. Nitrite levels were determined using the Griess assay. The rate of nitrite production was estimated from the total nitrite accumulated in culture supernatants between 3.5 h and 24 h of growth and normalized to the time and OD 600 (mean ± SD, n = 3, Student's t -test; **** P ≤ 0.00005). I. The growth profile of the Δ nos mutant was determined in cultures containing either 1 mM NaNO 3 , 1 mM NaNO 2 or 10 mM NH 4 Cl. Values represent mean ± SD (n = 3).
Techniques Used: Derivative Assay, Mutagenesis, Incubation, Drug Susceptibility Assay, Comparison, Positive Control, Concentration Assay, Fluorescence, Griess Assay
Related Articles
other:Article Title: The activation of lactate dehydrogenase induced by mTOR drives neoplastic change in breast epithelial cells Article Snippet: This assay was performed by using a Article Title: Galloflavin suppresses lactate dehydrogenase activity and causes MYC downregulation in Burkitt lymphoma cells through NAD/NADH-dependent inhibition of sirtuin-1 Article Snippet: Activation of the myc oncogene in cancer cells upregulates lactate dehydrogenase A (LDH-A) expression, leading to a sustained glycolytic flux that is needed to produce ATP under hypoxic conditions.. We studied the effects of galloflavin (GF), a recently identified LDH inhibitor, on myc overexpressing Burkitt lymphoma (BL) cells.. Epstein–Barr virus-infected lymphoblasts were used as a non-neoplastic control. Article Title: Metabolic activation triggered by cAMP in MCF-7 cells generates lethal vulnerability to combined oxamate/etomoxir. Article Snippet: Background: Altered energy metabolism is a biochemical fingerprint of cancer cells, widely recognized as one of the “hallmarks of cancer”.. Cancer cells show highly increased rates of glucose uptake and glycolysis, after which the resulting pyruvate is converted to lactate.. The maintenance of this metabolic asset is warranted by lactate dehydrogenase A (LDH-A) and for this reason the development of novel LDH-targeted anticancer therapeutics is underway. Article Title: Insights into pancreatic β cell energy metabolism using rodent β cell models Article Snippet: Although, the Fluorescence:Article Title: Hepatocytes determine the hypoxic microenvironment and radiosensitivity of colorectal cancer cells through production of nitric oxide that targets mitochondrial respiration. Article Snippet: This study explored the impact of hepatocytes on radiosensitivity of colorectal cancer in a model of diffusion-limited oxygenation relevant to liver metastases.. Because of their high respiratory rate, resting hepatocytes rapidly deplete oxygen and thereby radioprotect tumor cells.. Conversely, nitric oxide-producing hepatocytes reveal radiosensitizing properties, as the result of a respiratory self-arrest and reoxygenation of tumor cells. Article Title: LACK, a RACK1 ortholog, facilitates cytochrome c oxidase subunit expression to promote Leishmania major fitness Article Snippet: .. Briefly, whole cells were incubated with Electron Paramagnetic Resonance:Article Title: Hepatocytes determine the hypoxic microenvironment and radiosensitivity of colorectal cancer cells through production of nitric oxide that targets mitochondrial respiration. Article Snippet: This study explored the impact of hepatocytes on radiosensitivity of colorectal cancer in a model of diffusion-limited oxygenation relevant to liver metastases.. Because of their high respiratory rate, resting hepatocytes rapidly deplete oxygen and thereby radioprotect tumor cells.. Conversely, nitric oxide-producing hepatocytes reveal radiosensitizing properties, as the result of a respiratory self-arrest and reoxygenation of tumor cells. Control:Article Title: Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells. Article Snippet: Oxygen consumption measurements provide information on the activity of the mitochondrial electron transport chain and such measurements can be carried out on both isolated mitochondria and whole cells.. Additional information is available on glycolytic metabolism by assessing extracellular acidification.. The protocols in this unit describe how recently developed fluorescent pHand O2-sensitive probes can be used as tools for the assessment of mitochondrial toxicity and metabolic function in standard microtiter plates. Software:Article Title: Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells. Article Snippet: Oxygen consumption measurements provide information on the activity of the mitochondrial electron transport chain and such measurements can be carried out on both isolated mitochondria and whole cells.. Additional information is available on glycolytic metabolism by assessing extracellular acidification.. The protocols in this unit describe how recently developed fluorescent pHand O2-sensitive probes can be used as tools for the assessment of mitochondrial toxicity and metabolic function in standard microtiter plates. Incubation:Article Title: LACK, a RACK1 ortholog, facilitates cytochrome c oxidase subunit expression to promote Leishmania major fitness Article Snippet: .. Briefly, whole cells were incubated with |